The transition from the late Neolithic to the Early Bronze Age in Central Europe is a time period for which human mobility has been vividly debated in archaeological research. This presentation contributes to these considerations using an interdisciplinary approach that integrates ancient mitochondrial DNA analysis, the determination of stable isotope ratios of strontium and oxygen in tooth enamel, and archaeological analysis of radiocarbon dated skeletal remains. They represent 83 human individuals from 6 sites of the Bell Beaker Complex and the early Bronze Age in the Lech Valley in Southern Bavaria, Germany. Mitochondrial DNA analysis documented a diversification of haplogroups over time. Strontium and oxygen isotope ratios disclosed more than half of the females to be non-local, while there were only single occurrences among the male and subadult individuals. This striking pattern of patrilocality and female exogamy prevailed between about 2500 and 1700 BC. It was independent of individual sites and their archaeological assignments to the Bell Beaker Complex or the Early Bronze Age. While the males ensured settlement continuity in a spatially limited area, the results indicate that the females were driving forces for regional and supra-regional communication and exchange at the dawn of the European Metal Ages.